Unless specified otherwise, the articles and journal content published by MNK Publication are licensed under a Creative Commons Attribution license (CC-BY). This permits you to freely use, reproduce, and distribute the articles and related content for both commercial and noncommercial purposes, provided that the original source is properly cited in accordance with the CC-BY license. This content is covered under the Creative Commons Attribution 4.0 International License. Creative Commons Attribution 4.0 International License.

The title page should bear the title of the paper, the full names of all the authors and their affiliations, together with the name, full postal address, telephone and fax numbers and e-mail address of the author to whom correspondence and offprint requests are to be sent (this information is also asked for on the electronic submission form).

• The title should be brief, informative, of 150 characters or less and should not make a statement or conclusion.
• The running title should consist of no more than 50 letters and spaces. It should be as brief as possible, convey the essential message of the paper and contain no abbreviations.
• Authors should disclose the sources of any support for the work, received in the form of grants and/or equipment and drugs.
• If authors regard it as essential to indicate that two or more co-authors are equal in status, they may be identified by an asterisk symbol with the caption ‘These authors contributed equally to this work’ immediately under the address list.

Articles must be prepared with a structured abstract designed to summarise the essential features of the paper in a logical and concise sequence under the following mandatory headings

• Background/Objectives: What was the main question or hypothesis tested?
• Subjects/Methods: How many subjects were recruited, how many dropped out? Was the study randomised, case-controlled etc? Interventions/methods used and duration of administration.
• Results: Indicate 95% confidence intervals and exact P value for effects.
• Conclusions: Answer (significant or not) to main question.

The Introduction should assume that the reader is knowledgeable in the field and should therefore be as brief as possible but can include a short historical review where desirable.

This section should contain sufficient detail, so that all experimental procedures can be reproduced, and include references. Methods, however, that have been published in detail elsewhere should not be described in detail. Authors should provide the name of the manufacturer and their location for any specifically named medical equipment and instruments, and all drugs should be identified by their pharmaceutical names, and by their trade name if relevant.

The Results section should briefly present the experimental data in text, tables or figures. Tables and figures should not be described extensively in the text, either. The discussion should focus on the interpretation and the significance of the findings with concise objective comments that describe their relation to other work in the area. It should not repeat information in the results. The final paragraph should highlight the main conclusion(s), and provide some indication of the direction future research should take.

You should also acknowledge direct technical assistance, including help with animals, cells, equipment, patients, procedures, or techniques or provision of data, equipment, reagents, or samples, as well as more indirect assistance via intellectual discussions. Note that all of these contributions are typically more mechanical, indirect, and/or one-dimensional than those of authors. Additionally, some argue that individuals who provided help and could be chosen as a peer reviewer, leading to a potential conflict of interest, should be cited.

Authors must declare whether or not there are any competing financial interests in relation to the work described. This information must be included at this stage and will be published as part of the paper. Conflict of interest should be noted in the cover letter and also on the title page. Pl ease see the Conflict of Interest documentation in the Editorial Policy section for detailed information.

Only papers directly related to the article should be cited. Exhaustive lists should be avoided. References should follow the Vancouver format. In the text they should appear as numbers starting at one and at the end of the paper they should be listed (double-spaced) in numerical order corresponding to the order of citation in the text. Where a reference is to appear next to a number in the text.

Example.

1. Hoppert M. Microscopic techniques in biotechnology. Weinheim: Wiley-VCH; 2003.
2. Drummond PD. Triggers of motion sickness in migraine sufferers. Headache. 2005;45(6):653-6.
3. Meltzer PS, Kallioniemi A, Trent JM. Chromosome alterations in human solid tumors. In: Vogelstein B, Kinzler KW, editors. The genetic basis of human cancer. New York: McGraw-Hill; 2002. p. 93-113.
4. Storey KB, editor. Functional metabolism: regulation and adaptation. Hoboken (NJ): J. Wiley & Sons; 2004.
5. Halpern SD, Ubel PA, Caplan AL. Solid-organ transplantation in HIV-infected patients. N Engl J Med. 2002;347(7):284-7.

Only necessary illustrations should be included. All illustrations (line drawings and photographs) are classified as figures. Figures should be cited in consecutive order in the text. Figures should be sized to fit within the column or the full text width. Line figures should be supplied as sharp, black and white or color diagrams, drawn with a computer graphics package. Photographs should be sharp and magnifications should be indicated on photographs using a scale bar. Graphics should be supplied as high-resolution (at least 300 d.p.i.) electronic files. Digital images supplied as low-resolution cannot be used and will not be accepted. The legend should incorporate definitions of any symbols used and all abbreviations and units of measurement should be explained so that the figure can be understood without reference to the text.

Tables should be self-contained and the data should not be duplicated in figures. Tables should be numbered consecutively. Each table should be presented on a separate page with a comprehensive but concise legend above the table. Tables should be double-spaced and vertical lines should not be used to separate columns. Column headings should be brief, with units of measurement in parentheses. All abbreviations should be defined in footnotes

SI units as outlined in the latest edition of Units, symbols and Abbreviations: A Guide for Medical and Scientific Editors and Authors (Royal Society of Medicine Press, London), should be used wherever possible. Statistics and measurements should always be given in figures; except where the number begins the sentence. When the number does not refer to a unit measurement, it is spelt out, except where the number is greater than nine. Use only standard abbreviations. The word Figure should be shortened to Fig. unless starting a sentence.

The authors should acknowledge the funders of this manuscript and provide all necessary funding information.