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International Journal of Latest Research in Science and Technology

DOI:10.29111/ijlrst   ISRA Impact Factor:3.35,  Peer-reviewed, Open-access Journal

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HAPLOTYPE ANALYSIS OF SINGLE NUCLEOTIDE POLYMORPHISMS OF CYP1A1 AND CYP1A2 AND ITS RELATIONSHIP WITH THE DEVELOPMENT OF PORPHYRIA CUTANEA TARDA.

Research Paper Open Access

International Journal of Latest Research in Science and Technology Vol.7 Issue 6, pp 12-17,Year 2018

HAPLOTYPE ANALYSIS OF SINGLE NUCLEOTIDE POLYMORPHISMS OF CYP1A1 AND CYP1A2 AND ITS RELATIONSHIP WITH THE DEVELOPMENT OF PORPHYRIA CUTANEA TARDA.

Diego Miguel Gordillo*, Lubna Abou Assali*, Gabriela Nora Cerbino, Laura Sabina Varela, Alcira Batlle, Victoria Estela Parera, María Victoria Rossett

Received : 21 December 2018; Accepted : 03 January 2019 ; Published : 29 January 2019

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Article No. 10961
Abstract

Objective: Porphyria Cutanea Tarda results from decrease hepatic uroporphyrinogen decarboxylase activity. There are two principal types: sporadic or acquired and familial or hereditary Porphyria Cutanea Tarda. Hereditary Porphyria Cutanea Tarda is observed in 25-30% of the patients in whom one allele of the uroporphyrinogen decarboxylase gene reduces the enzyme activity by approximately 50% in all tissues. There are experimental evidences that suggest a possible relationship between CYP1A1, CYP1A2 and Porphyria Cutanea Tarda symptomatology development but the results described in different populations are very conflictive.The aim of this work is provide additional evidences about the participation of the CYP system on the manifestation of this disease. Subjets/Methods: We analyse the polymorphisms already described in these CYPs isoforms in 112 Porphyria Cutanea Tarda Argentinian patients and 89 controls. Of these 64 were used for haplotype analysis. The polymorphisms were detected by RFLP-PCR or sequencing. Results: For CYP1A2*1F polymorphism C allele (wt) was exceptionally the less frequent while the polymorphic A allele, with an increased transcriptional activity, resulted to be the risk allele. For CYP1A1 m4 polymorphism, for which are not previous reports about this effect, our results indicated that the A variant would be a risk factor to develop the disease. For m2 polymorphism we obtained contradictory results depending on the groups compared. The risk haplotype calculated with the snpSTATs was m4-m2-m1-1A2: C-G-C-C. Conclusions: These results indicated that, among other factors, these polymorphisms would be related with Porphyria Cutanea Tarda development.

Key Words   
CYPs,PCT,SNPs,URO-D

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To cite this article

Diego Miguel Gordillo*, Lubna Abou Assali*, Gabriela Nora Cerbino, Laura Sabina Varela, Alcira Batlle, Victoria Estela Parera, María Victoria Rossett , " Haplotype Analysis Of Single Nucleotide Polymorphisms Of Cyp1a1 And Cyp1a2 And Its Relationship With The Development Of Porphyria Cutanea Tarda. ", International Journal of Latest Research in Science and Technology . Vol. 7, Issue 6, pp 12-17 , 2018

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