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International Journal of Latest Research in Science and Technology

DOI:10.29111/ijlrst   ISRA Impact Factor:3.35,  Peer-reviewed, Open-access Journal

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λ-SCHISANDRIN INHIBITS EPITHELIAL-MESENCHYMAL TRANSITION OF HUMAN RENAL TUBULAR EPITHELIAL HK-2 CELLS INDUCED BY TRANSFORMING GROWTH FACTOR-β1

Research Paper Open Access

International Journal of Latest Research in Science and Technology Vol.6 Issue 1, pp 61-65,Year 2017

λ-SCHISANDRIN INHIBITS EPITHELIAL-MESENCHYMAL TRANSITION OF HUMAN RENAL TUBULAR EPITHELIAL HK-2 CELLS INDUCED BY TRANSFORMING GROWTH FACTOR-β1

Shuang Li, Ying Zhong, Xiao-meng Dai, Pei-di Yin, Bin Du

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Received : 23 February 2017; Accepted : 25 February 2017 ; Published : 28 February 2017

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Article No. 10711
Abstract

Renal injury and renal fibrosis are closely associated with the progression of chronic kidney disease (CKD). The epithelial-to-mesenchymal transition (EMT) takes an indispensable place of human tubular epithelial cells in renal interstitial. The transforming growth factor TGF- ß1 induced EMT in human kidney proximal tubular epithelial cell line (HK-2 cells). At the same time, the morphology of HK-2 cells altered along with the change of treated methods clearly. These evidences suggest the abduction of EMT by TGF- ß1. Although the involvement in several physiological function of γ-Schisandrin had been discovered, the medical function of TGF- ß1-induced renal fibrosis is still undiscovered. As important elements of EMT, the increased expression of collagen type Ⅰand Ⅳ decreased significantly after pre-treated with γ-Schisandrin. In addition, the gene expression of Snail, which represents myofibroblast, reduced obviously. In the present investigation, it was suggested that Sch might be capable to antagonize TGF- ß1-induced renal fibrosis and EMT in HK-2 cells. Overall, the application of traditional Chinese medicine (Sch) for the treatment of renal fibrosis patients provides an original therapeutic ideas and bright prospects to patients families.

Key Words   
Epithelial-to-mesenchymal transition (EMT), renal fibrosis, HK-2 cell, TGF- ß1, γ-Schi

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To cite this article

Shuang Li, Ying Zhong, Xiao-meng Dai, Pei-di Yin, Bin Du , " λ-schisandrin Inhibits Epithelial-mesenchymal Transition Of Human Renal Tubular Epithelial Hk-2 Cells Induced By Transforming Growth Factor-β1 ", International Journal of Latest Research in Science and Technology . Vol. 6, Issue 1, pp 61-65 , 2017

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