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International Journal of Latest Research in Science and Technology

DOI:10.29111/ijlrst   ISRA Impact Factor:3.35,  Peer-reviewed, Open-access Journal

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STRUCTURAL EVIDENCE OF RECEPTOR MEDIATED ENDOCYTOSIS AT POSTERIOR LENS SUTURES

Research Paper Open Access

International Journal of Latest Research in Science and Technology Vol.3 Issue 6, pp 68-73,Year 2014

STRUCTURAL EVIDENCE OF RECEPTOR MEDIATED ENDOCYTOSIS AT POSTERIOR LENS SUTURES

Kristin J. Al-Ghoul,Sean T. Donohue

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Received : 17 December 2014; Accepted : 25 December 2014 ; Published : 31 December 2014

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Article No. 10423
Abstract

Purpose: To investigate whether lens sutures have structural parameters (clathrin coated vesicles) to support enriched transport of metabolites into the lens via receptor mediated endocytosis. Methods: Lenses from 20 normal 6 week old rats and 6 adult chickens were utilized for this study. A transmission electron microscopic (TEM) survey was conducted to establish the presence of coated vesicles/pits within suture planes and at apical and basal ends of elongating fibers. Coated vesicle density was quantified both within and outside sutural domains. Clathrin was localized by fluorescent immunolabeling and laser scanning confocal microscopic (LSCM) visualization in both rat and chicken lenses. Results: TEM examination demonstrated that abundant coated vesicles were present at the epithelial-fiber interface (EFI) in the apical domains of both epithelial cells and elongating fibers. Quantification revealed that although coated vesicle density was greater within than without anterior sutural domains, the difference was not significant. Abundant coated vesicles were also found at basal ends of elongating fibers, however their density was significantly greater within posterior sutural domains as compared to off-suture locations (p<0.001). Statistical comparisons of anterior vs. posterior locations showed that the density of coated vesicles was significantly different in both on- and off-suture locations. Immunolocalization demonstrated that clathrin was abundant at both anterior and posterior fiber ends in elongating fibers. Clathrin immunofluorescence was more pronounced at posterior sutures than at anterior sutures. This distribution was consistent in both rat and chicken lenses. Conclusions: Posterior lens sutures appear to facilitate transport of metabolites from the vitreous into lens fibers. Although suture planes are detrimental to lens optics, their anatomical arrangement along the visual axis may be important for optimal lens function. The lower amount of clathrin at anterior as opposed to posterior sutures is probably a result of the high transport capability of the anterior lens epithelium.

Key Words   
Crystalline Lens, Clathrin, Coated Vesicles, Receptor Mediated Endocytosis, Immunohistoche

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References
  1. Kuszak, J. R., M. Mazurkiewicz, and R. Zoltoski. 2006. Computer modeling of secondary fiber development and growth: I. Nonprimate lenses. Mol.Vis. 12:251-270.
  2. Lo, W.-K. and W. Zhang. 1989. Endocytosis of Macromolecules in the Lenses of Guinea Pig and Rabbit. Lens Eye Toxic.Res., 6:603-612.
  3. Brown, H. G., M. Ireland, G. D. Pappas, and J. R. Kuszak. 1990. Ultrastructural, biochemical and immunological evidence of receptor-mediated endocytosis in the crystalline lens. Invest.Ophthalmol.& Visual Sci., 31:2579-2592.
  4. Lo, W. K., A. Mills, W. Zhang, and H. Zhu. 1991. Polarized distribution of coated pits and coated vesicles in the rat lens: an electron microscopy and WGA-HRP trace study. Current Eye Research 10(12):1151-1163.
  5. Bassnett, S., J. R. Kuszak, L. Reinisch, H. G. Brown, and D. C. Beebe. 1994. Intercellular communication between epithelial and fiber cells of the eye lens. J.Cell Sci., 107 (4):799-811.
  6. Kuszak, J. R. and H. G. Brown. 1994. Embryology and anatomy of the crystalline lens, p. 82-96. In D. A. Albert and F. A. Jakobiec (ed.), Principles and Practice of Ophthalmology: Basic Sciences. W.B. Saunders Company, Philadelphia.
  7. Kuszak, J. R., L. A. Novak, and H. G. Brown. 1995. An Ultrastructural Analysis of the Epithelial-Fiber Interface (EFI) in Primate Lenses. Exper.Eye Res. 61:579-597.
  8. Boyle, D. L., P. Carmen, and L. Takemoto. 2002. Translocation of Macromolecules into Whole Rat Lenses in Culture. Mol Vis 10:226-234.
  9. Zhou, C. and W. K. Lo. 2003. Association of Clathrin, AP-2 Adaptor and Actin Cytoskelton with Developing Interlocking Membrane Domains of Lens Fibre Cells. Exp Eye Res 77:423-432.
  10. Sabah, J., B. Schultz, Z. Brown, A. Nguyen, J. R. Reddan, and L. J. Takemoto. 2007. Transcytotic passage of albumin through lens epithelial cells. Invest Ophthalmol Vis Sci 48:1237-1244.
  11. Kuszak, J. R., J. G. Sivak, and J. A. Weerheim. 1991. Lens optical quality is a direct function of lens sutural architecture. Invest.Ophthalmol.& Vis.Sci. 32, No. 7:2119-2129.
  12. Kuszak, J. R., K. L. Peterson, K. L. Herbert, and J. G. Sivak. 1994. The Inter-Relationship of Lens Anatomy and Optical Quality.   Primate Lenses. Exper.Eye Res. 59:521-535.
  13. Sivak, J. G., K. L. Herbert, K. L. Peterson, and J. R. Kuszak. 1994. The interrelationship of lens anatomy and optical quality. I. Non-primate lenses. Exper.Eye Res. 59:505-520.
  14. Gorthy, W. C. and J. W. Anderson. 1980. Special characeristics of the polar regions of the rat lens: morphology and phosphatase histochemistry. Investigative Ophthalmology and Visual Science 19 (9):1038-1052.
  15. Benedetti, E. L., I. Dunia, C. J. Bentzel, A. J. M. Vermorken, M. Kibbelaar, and H. Bloemendal. 1976. A portrait of plasma membrane specializations in lens epithelium and fibers. Biochim.et Biophys.Acta. 457:353-384.
  16. Goodenough, D. A., J. S. B. I. Dick, and J. E. Lyons. 1980. Lens metabolic cooperation: a study of mouse lens transport and permeability visualized with freeze-substitution autoradiography and electron microscopy. J.Cell Biol. 86:576-589.
  17. Gorthy, W. C., M. R. Snavely, and N. D. Berrong. 1971. Some aspectes of transport and digestion in the lens of the normal young adult rat. Exper.Eye Res. 12:112-119.
  18. Lo, W. K., C. Zhou, and J. Reddan. 2004. Identification of caveolae and their signature proteins caveolin 1 and 2 in the lens. Exp Eye Res 79:487-498.
  19. Dahm, R., J. van Marle, R. A. Quinlan, A. R. Prescott, and G. F. J. M. Vrensen. 2011. Homeostasis in the vertebrate lens: mechanisms of solute exchange. Phil.Trans.R.Soc.B 366:1265-1277.
To cite this article

Kristin J. Al-Ghoul,Sean T. Donohue , " Structural Evidence Of Receptor Mediated Endocytosis At Posterior Lens Sutures ", International Journal of Latest Research in Science and Technology . Vol. 3, Issue 6, pp 68-73 , 2014

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