Objectives: To assess Sudanese dentist’s knowledge, Attitude and Practice towards dental waste disposal management. Subjects and Methods: A descriptive cross-sectional study among 200 dentists of whom 51.5% were males and 48.5% were females (general practitioners 68.5% and 31.5% specialists) working in private clinics in Khartoum locality. Self-administered questionnaire including questions about demographic data, years of experience, specialty and questions regarding the knowledge, attitude and practice towards dental waste management. The resulting data were statistically tested using chi-square test for differences between variables with P value ≤ 0.05 indicating level of significance. Results: Completed questionnaires were returned anonymously. The response rate was 82.5%. The majority (96.4%) knew about the harmful effect of dental waste disposal, 97.6% were aware about the transmissible infectious diseases (HIV/ Hepatitis B&C) and 95.2% said that waste should be segregated into categories. The majority (84.2%) of the respondents would like to attend a program on dental waste management, 17.6% agreed that health care is not an issue, yet 76.4% stated that it’s a financial burden on the clinic and 40% stated that it's an extra work burden. There was statistical significant association between practice and gender/specialty (general or specialist) regarding specific waste disposal techniques (P < 0.05). Also statistical significant association was found between attitude and gender/year of experience with P = 0.049 and 0.001 respectively. Conclusion: Majority of dentists working in private clinics are knowledgeable about dental waste management. The attitude towards dental waste segregation is affected by the financial burden yet there is a need for training programs with an implementation of an annual audit and education within the dental health care centers.