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International Journal of Latest Research in Science and Technology

DOI:10.29111/ijlrst   ISRA Impact Factor:3.35,  Peer-reviewed, Open-access Journal

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INSULIN GENE EXPRESSION AND ITS DETECTION IN THE RAT KIDNEY

Research Paper Open Access

International Journal of Latest Research in Science and Technology Vol.3 Issue 3, pp 194-200,Year 2014

INSULIN GENE EXPRESSION AND ITS DETECTION IN THE RAT KIDNEY

Claudia Soto, Jesús Juárez, Julia Pérez, Imelda González,Ana Esquivel, Esther Uría, Salud Pérez, Luis Raya

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Received : 26 June 2014; Accepted : 28 June 2014 ; Published : 30 June 2014

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Article No. 10340
Abstract

Insulin is not only produced by highly specific β pancreatic cells, it is currently known that there are extrapancreatic tissues expressing insulin mRNA such as the brain, thymus and some cells in the olfactory mucosa. Pdx-1 and Nkx6.1 are key transcription factors expressed during pancreatic development, differentiation and maintenance of beta cell function as well as in the synthesis of insulin. These transcription factors are also found in the brain. In this study we analyze the genic expression of insulin Pdx-1 and Nkx6.1 in the rat kidney. We investigated its genic expression by qRT-PCR assay and were confirmed by sequencing the qRT-PCR products. Renal cDNA, was assayed in parallel with rat pancreatic cDNA. Insulin, Pdx-1 and Nkx6.1 were analyzed in the renal tissue by immunohistochemistry. Pdx-1 and Nkx6.1 were also assayed by immunoblot. The resemblance percentages for kidney insulin, Pdx-1 and Nkx6.1 were, 100, 97 and 98% respectively compared to pancreatic insulin and transcription factors. The relative expression level for insulin was lower in the kidney than in the pancreatic tissue. Insulin and its transcription factors were immunodetected in the rat kidney tissue. These results suggest the expression and detection of insulin, Pdx-1 and Nkx6.1. Both transcription factors are involved in the synthesis of insulin. Our findings may be related with insulin function at renal level. However, further research is necessary in order to conclusively prove it. At the renal level, insulin promotes renal sodium reabsorption, participates in the decrease of potassium excretion and renal vascular resistance.

Key Words   
Insulin, Pdx-1, Nkx6.1, kidney tubules

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To cite this article

Claudia Soto, Jesús Juárez, Julia Pérez, Imelda González,Ana Esquivel, Esther Uría, Salud Pérez, Luis Raya , " Insulin Gene Expression And Its Detection In The Rat Kidney ", International Journal of Latest Research in Science and Technology . Vol. 3, Issue 3, pp 194-200 , 2014

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