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International Journal of Latest Research in Science and Technology

DOI:10.29111/ijlrst   ISRA Impact Factor:3.35,  Peer-reviewed, Open-access Journal

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PARKIN, PARKINSON DISEASE GENE PRODUCT, DIRECTLY REDUCES HYDROGEN PEROXIDE (MITOCHONDRIAL OXIDANT), AND FORMS DIMERIZATION REVERSIBLY.

Research Paper Open Access

International Journal of Latest Research in Science and Technology Vol.5 Issue 4, pp 1-3,Year 2016

PARKIN, PARKINSON DISEASE GENE PRODUCT, DIRECTLY REDUCES HYDROGEN PEROXIDE (MITOCHONDRIAL OXIDANT), AND FORMS DIMERIZATION REVERSIBLY.

Tohru Kitada ,Rie Hikita, Hideo Hirose

Correspondence should be addressed to :

Received : 01 July 2016; Accepted : 21 July 2016 ; Published : 31 August 2016

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Article No. 10665
Abstract

The current central dogma of Parkin function is that the E3 ligase Parkin ubiquitinates its substrate on the surface of damaged mitochondria and removes them to maintain mitochondrial quality. However, recent pathological and biochemical evidence confirmed that Parkin has a protective function from oxidative stress, especially in mitochondria. The relationship between the two hypotheses has not yet been clear. In this short report, we show; First, Parkin reacts with H2O2 directly and reduces it, and turns into Parkin dimer. Furthermore, supplied antioxidant changes dimer into monomer again. These suggest that Parkin is also a redox molecule in mitochondria as well as E3 ligase outside mitochondria.

Key Words   
Parkin, PINK1, DJ-1, Familial Parkinson’s Disease, Mitochondria, Oxidative Stress, E3 Ligase, Hydr
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References
  1. Kitada T, Asakawa S, Hattori N, Matsumine H, Yamamura Y, Minoshima S, Yokochi M, Mizuno Y, Shimizu N. Mutations in the parkin gene cause autosomal recessive juvenile parkinsonism. Nature. 392(6676):605-608 (1998).
  2. Narendra DP, Jin SM, Tanaka A, Suen DF, Gautier CA, Shen J, Cookson MR, Youle RJ. PINK1 is selectively stabilized on impaired mitochondria to activate Parkin. PLoS Biol. 8(1):e1000298. doi: 10.1371/journal.pbio.1000298 (2010).
  3. Meng F, Yao D, Shi Y, Kabakoff J, Wu W, Reicher J, Ma Y, Moosmann B, Masliah E, Lipton SA, Gu Z. Oxidation of the cysteine-rich regions of parkin perturbs its E3 ligase activity and contributes to protein aggregation. Mol Neurodegener. 6:34. doi:10.1186/1750-1326-6-34 (2011).
  4. Taira T, Saito Y, Niki T, Iguchi-Ariga SM, Takahashi K, Ariga H. DJ-1 has a role in antioxidative stress to prevent cell death. EMBO Rep. 5(2):213-218 (2004).
  5. Palacino JJ, Sagi D, Goldberg MS, Krauss S, Motz C, Wacker M, Klose J, Shen J. Mitochondrial dysfunction and oxidative damage in parkin-deficient mice. J Biol Chem. 279(18):18614-18622 (2004).
  6. Gautier CA, Kitada T & Shen J. Loss of PINK1 causes mitochondrial functional defects and increased sensitivity to oxidative stress. Proc Natl Acad Sci U S A.105:11364-11369 (2008).
  7. Takanashi M, Mochizuki H, Yokomizo K, Hattori N, Mori H, Yamamura Y, Mizuno Y. Iron accumulation in the substantia nigra of autosomal recessive juvenile parkinsonism (ARJP). Parkinsonism Relat Disord. 7(4):311-314 (2001).
  8. LaVoie MJ, Ostaszewski BL, Weihofen A, Schlossmacher MG & Selkoe DJ. Dopamine covalently modifies and functionally inactivates parkin. Nature medicine. 11:1214-1221 (2005).
  9. Nulton-Persson AC, Szweda LI. Modulation of mitochondrial function by hydrogenperoxide. J Biol Chem. 276(26):23357-23361 (2001).
  10. Kuroda Y, Sako W, Goto S, Sawada T, Uchida D, Izumi Y, Takahashi T, Kagawa N, Matsumoto M, Matsumoto M, Takahashi R, Kaji R, Mitsui T. Parkin interacts with Klokin1 for mitochondrial import and maintenance of membrane potential. Hum Mol Genet. 21(5):991-1003 (2012).
  11. LaVoie MJ, Cortese GP, Ostaszewski BL, Schlossmacher MG. The effects ofoxidative stress on parkin and other E3 ligases. J Neurochem. 103(6):2354-2368 (2007).
  12. Chaugule VK, Burchell L, Barber KR, Sidhu A, Leslie SJ, Shaw GS, Walden H. Autoregulation of Parkin activity through its ubiquitin-like domain. EMBO J. 30(14):2853-2867 (2011).
To cite this article

Tohru Kitada ,Rie Hikita, Hideo Hirose , " Parkin, Parkinson Disease Gene Product, Directly Reduces Hydrogen Peroxide (mitochondrial Oxidant), And Forms Dimerization Reversibly. ", International Journal of Latest Research in Science and Technology . Vol. 5, Issue 4, pp 1-3 , 2016

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