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International Journal of Latest Research in Science and Technology

DOI:10.29111/ijlrst   ISRA Impact Factor:3.35

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ANTIBIOTIC RESISTANCE AND PRODUCTION OF ENTEROTOXINS OF STAPHYLOCOCCUS SPP. ISOLATED FROM SAMPLES OF MILK IN DAIRY SHEEP

Research Paper Open Access

International Journal of Latest Research in Science and Technology Vol.3 Issue 6, pp 99-102,Year 2014

ANTIBIOTIC RESISTANCE AND PRODUCTION OF ENTEROTOXINS OF STAPHYLOCOCCUS SPP. ISOLATED FROM SAMPLES OF MILK IN DAIRY SHEEP

František Zigo,Milan Vasiľ, Juraj Elečko,Zuzana Farkašová,Martina Chripková

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Received : 02 December 2014; Accepted : 14 December 2014 ; Published : 31 December 2014

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Abstract

Milk samples from 450 sheep of Lacaune breed with machine milking were collected two times during one lactation season. After microbiological examinations and in vitro test of resistance to antibiotics, by PCR was determined presence of genes coding production of enterotoxins, and by Elisa methods production individual types of enterotoxins. The results suggested on higher occurrence of subclinical (5.9 %) and subacute (4.8 %) forms of mastitis. The coagulase-negative staphylococci (CNS), and S. aureus were identified in 103 (70 %) from all 147 positive isolates. Besides to higher resistance to Oxacilin, Cloxacilin and Penicillin were determined in S. aureus, S. schleiferi, S. epidermidis and S. chromogenes the production of staphylococcal enterotoxins (SE) of type A, B, C and D.

Key Words   
Dairy sheep,Lacaune breed,Mastitis,Resistance,Staphylococcal enterotoxins

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References
  1. Beatriz, M., Borelli, E.G., Ferreira, I.C., Lacerda, D.A., Santos, L.S., Carmo, R.S., Dias, M., Crisolita, C., Silva, C.A. (2006). Enteroxigenic Staphylococcus and other microbial contaminants during production of Canastra cheese, Brazil. Braz. J. Microbiol. 37(4):545-550.
  2. Becker, K., Keller, B., Von, E.C., Brück, M., Lubritz, G., Etienne, J., Peters, G. (2001). Enterotoxigenic potential of Staphylococcus intermedius. Environ. Microbiol. 67:5551-5557.
  3. Becker, K., Roth, R., Peters, G. (1998). Rapid and specific detection of toxigenic Staphylococcus aureus: use of two multiplex PCR enzyme immunoassays for ammplification and hybridization of staphylococcal enterotoxin genes, exfoliative toxin genes, and toxic syndrome toxin-1 gene. Clin. Microbiol. 36:2548-2553.
  4. Bergonier, D., Crémoux, R., Rupp, R., Lagriffoul, G. Erthelot, X. (2003). Mastitis of dairy small ruminants. Res. 34:689-716.
  5. Berthelot, X., Lagriffoul, G., Concordet, D., Barillet, F., Bergonier, D. (2006). Physiological and pathological thresholds of somatic cell counts in ewe milk. Small Ruminant Research. 62:27–31.
  6. Conington, J., Cao, G., Stott, A., Bůnger, L. (2008). Breeding for resistance to mastitis in United Kingdom sheep, a review and economic appraisal. Veterinary Record. 162:369-376.
  7. Contreras, D., Sierra, A., Sanchez, J. C., Corrales, J. C., Marco, M.J., Paape, C., Gonzalo, A. (2007). Mastitis in small ruminants. Small Ruminant Research, Special Issue: Goat and Sheep Milk. 68, (1–2):145–153.
  8. Fthenakis, G.C. (1994). Prevalence and aetiology of subclinical mastitis in ewes of southern Greece. Small Rumin. Res. 13:293–300.
  9. Fthenakis, G.C. (1995). California mastitis test and Whiteside test in diagnosis of subclinical mastitis of dairy ewes. Small Ruminant Res. 16:271–276.
  10. Hariharan, H., Donachie, W., Macaldowie, C., Keefe, G. (2004). Bacteriology and somatic cell counts in milk samples from ewes on a Scottish farm. J. Vet. Res. July 68(3):188–192.
  11. Hisira, V., Farkašova, Z., Elečko, J., Mudroň, P. (2013). The occurrence of subclinical mastitis in dairy farming with deep litter. Slovac Veterinary Journal. 38:270-273.
  12. Malinowski, E., Lassa, H., Kŀossowska, A., Smulski, S., Markiwicz, H., Kaczmarowski, M. (2006). Etiological agents of dairy cows’ mastitis in western part of Poland.  Jour. Vet. Sci. 9:191-194.
  13. Matsunaga, T., Kamata, S., Kakiichi, N., Uccida, A. (1993). Characteristics of Staphylococcus aureus isolated from peracute, acute and chronic bovine mastitis. Vet. Med. Sci. 55:297–300.
  14. Ozenc, E., Seker, E., Baki Acar, D., Birdane, M.K., Darbaz, I., Dogan, N. (2011). The Importance of Staphylococci and threshold value of somatic cell count for diagnosis of sub-clinical mastitis in pirlak sheep at mid-lactation. Dom. Anim. ISSN 0936-6768. 46:970–974.
  15. Sawant, A.A., Gillespie, B.E., Oliver, S.P. (2009). Antimicrobial susceptibility of coagulase-negative Staphylococcus species isolated from bovine milk. Microbiol. 134:73-81.
  16. Valle, J., Gomez, L.E., Piriz, S., Goyache, J., Orden, J.A., Vadilo, S. (1990). Enterotoxins production by Staphylococcal isolated from healthy goats. Applied and Environmental Microbiology. 56(5):1323-1326.
  17. Vautor, E., Cocckfield, J., Marechal Le, C., Le Loir, Y., Chevalier, M., Robinson, A.D., Thiery, R., Lindsay, J. 2009. Difference in virulence between Staphylococcus aureus isolates causing gangrenous mastitis versus subclinical mastitis in a dairy sheep flock. Res. 6:40 – 56.
  18. Zschöck, M., Botzler, D., Blöcher, S., Sommerhauser, J., Hamann, H.P. (2000). Detection of genes for enterotoxins (ent) and toxic shock syndrome toxin-1 (tst) in mammary isolates of Staphylococcus aureus by polymerase-chain-reaction. Int. Dairy J. 10:569–574.
  19. Zschöck, , Kloppert, W., Wolter, H., Hamann, P., Lämmler, CH. (2005). Pattern of enterotoxin genes seg, seh, sei and sej positive Staphylococcus aureus isolated from bovine mastitis, Veterinary Microbiology. 108(3–4):243–249.
To cite this article

František Zigo,Milan Vasiľ, Juraj Elečko,Zuzana Farkašová,Martina Chripková , " Antibiotic Resistance And Production Of Enterotoxins Of Staphylococcus Spp. Isolated From Samples Of Milk In Dairy Sheep ", International Journal of Latest Research in Science and Technology . Vol. 3, Issue 6, pp 99-102 , 2014

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